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目的研究干酪乳杆菌LC2W对胃上皮细胞MKN-45的粘附性质,探讨粘附与其表面性质的关系,初步判断粘附素的性质。方法通过化学和酶处理LC2W细胞壁表面成分,测定其粘附性质、表面性质的变化,并通过相关性分析粘附与表面性质的关系。结果氯化锂、胃蛋白酶、蛋白酶K、苯酚和热处理能显著降低LC2W的粘附性,表明表面的相关蛋白类物质可能参与了LC2W对MKN-45细胞的粘附。化学和酶处理后疏水能力和自聚合能力的变化也表明表面蛋白类物质的存在。相关性分析发现粘附能力分别与疏水性和自聚合能力呈现强正线型相关,证明蛋白类成分在粘附过程中发挥作用。结论 LC2W的表面粘附素是一种蛋白类物质。
Objective To study the adhesion properties of Lactobacillus casei LC2W to gastric epithelial cells MKN-45 and to explore the relationship between the adhesion and its surface properties, and to determine the properties of adhesins. Methods The surface of LC2W cell wall was chemically and enzymatically treated to determine its adhesion and surface properties. The relationship between the adhesion and surface properties was analyzed by correlation analysis. Results Lithium chloride, pepsin, protease K, phenol and heat treatment could significantly reduce the adhesion of LC2W, indicating that the surface-related proteins may be involved in the adhesion of LC2W to MKN-45 cells. Changes in hydrophobicity and self-polymerization ability after chemical and enzymatic treatment also indicate the presence of surface protein species. Correlation analysis showed that the adhesion ability showed a strong positive linear correlation with the hydrophobicity and self-polymerization ability respectively, which proves that the protein components play a role in the adhesion process. Conclusion The surface adhesin of LC2W is a proteinaceous material.