论文部分内容阅读
采用HPLC法测定血清中司帕沙星的浓度。血清标本经甲醇沉淀蛋白,高速离心后取上清液直接进样。色谱条件以PE-pack C_(18)柱(5μm,4.6mm×150mm)为色谱柱,甲醇-0.2mol·L~(-1)乙酸铵溶液(pH2.7)(30:70)为流动相,检测波长为298nm血清中药物的最低检测浓度为0.04μg·ml~(-1)。在0.05～2μg·ml~(-1)血药浓度范围内峰高与药物浓度线性关系良好,Y=5.835 1X-0.030 6,r=0.999 3。相对回收率为91.9％～103.4％,绝对回收率为91.5％～93.6％。日内RSD为1.5％～1.9％,日间RSD为1.9％～4.5％。方法简单、快速、准确,可用于司帕沙星的临床药动学研究。
Serum sparfloxacin concentration was determined by HPLC. Serum samples were precipitated by methanol, the supernatant was centrifuged and then directly injected. The chromatographic conditions were as follows: PE-pack C_ (18) column (5μm, 4.6mm × 150mm) column and methanol-0.2mol·L -1 ammonium acetate solution , The detection wavelength of 298nm serum drug detection minimum concentration of 0.04μg · ml ~ (-1). There was a good linear relationship between peak height and drug concentration in the range of 0.05 ~ 2μg · ml ~ (-1) plasma concentration, Y = 5.835 1X-0.030 6, r = 0.999 3. The relative recoveries ranged from 91.9% to 103.4%, and the absolute recoveries ranged from 91.5% to 93.6%. Intraday RSD was 1.5% ~ 1.9%, daytime RSD was 1.9% ~ 4.5%. The method is simple, rapid and accurate and can be applied to the clinical pharmacokinetics of sparfloxacin.