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现知B淋巴细胞表面具有膜免疫球蛋白(SIg)、Fc受体与补体受体,为此建立了相应的体外检测方法,其中以测定SIg的方法较为多用,但需借助荧光显微镜检查,推广时受到仪器的限制。测定补体受体的EAC花环试验虽然不能检出全部B细胞,但其优点是操作简便,不需特殊仪器,易于推广。如果操作方法恒定,可得重复结果,作为B细胞计数的方法仍为许多实验室所乐用。 EAC花环试验中,“E”指红细胞,“A”指抗红细胞抗体,“C”指补体。因带补体受体淋巴细胞(CRL)上的补体受体仅对补体C4b及C3裂解成分C3b、C3d呈特异性结合,因此测定补体受体必需要有活化的C3及证明C3与相应受体结合的指示系统。现知红细胞可与相应抗体呈特异性结合形成抗原抗体复合物(EA),后者通过补体传统途径活化补体而产生C3然后EA与C3结合形成EAC。凡带C3受体的淋巴细胞可通过C3受体与EAC结合形成花环,据此可计数淋巴细胞中的B细胞。 本文介绍一年来我室所用EAC-花环试验的方法、影响因素与点滴体会。
Now known B lymphocytes with membrane immunoglobulin (SIg), Fc receptors and complement receptors, thus establishing a corresponding in vitro detection methods, in which the determination of SIg is more versatile, but need to use fluorescence microscopy to promote When the instrument is limited. The EAC rosette assay, which measures complement receptors, does not detect all B cells but has the advantage of easy handling and ease of deployment without special instrumentation. If the method of operation is constant, repeatable results are obtained, and the method used for counting B cells is still used by many laboratories. EAC garland test, “E” refers to red blood cells, “A” refers to anti-red blood cell antibodies, “C” refers to complement. Because complement receptors on complement receptor lymphocytes (CRLs) specifically bind only to complement C4b and to the C3 cleavage components C3b and C3d, it is necessary to determine activated complement C3 and to prove that C3 binds to the corresponding receptor The indication system. Now known red blood cells can be combined with the corresponding antibodies specifically to form antigen-antibody complex (EA), which complement activation by complement the traditional way to produce C3 and then EA and C3 to form EAC. Lymphocytes with C3 receptors can bind to EAC via the C3 receptor to form a rosette, from which B cells in lymphocytes can be counted. This article describes the method used to test the EAC-garland used in my room over the past year, the influencing factors and the experience.