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目的检测Calcineurin/NFAT信号途径中Calcineurin,NFAT2,COX-2和VEGF-A分子在正常人黑素细胞和不同黑素瘤细胞系中的表达,初步探讨它们与黑素瘤发生、发展的关系。方法选取正常人原代黑素细胞(MC)及来源于原位(WM793B)、侵袭性(LIBR)及转移性(SK-MEL-5)的黑素瘤细胞系,采用RT-PCR,Westernblot检测四种细胞中Calcineurin,NFAT2,COX-2和VEGF-A的表达情况。结果与正常人黑素细胞相比,在不同黑素瘤细胞系中Calcineurin,NFAT2,COX-2和VEGF-A4个分子明显高表达。结论 Calcineurin/NFAT信号途径可能与黑素瘤的发生有关。
Objective To detect the expression of Calcineurin, NFAT2, COX-2 and VEGF-A in normal human melanocytes and different melanoma cell lines in Calcineurin / NFAT signaling pathway and to explore their relationship with the occurrence and development of melanoma. Methods Normal melanocytes (MCs) and melanoma cell lines derived from in situ (WM793B), invasive (LIBR) and metastatic (SK-MEL-5) cells were selected and detected by RT-PCR and Western blot The expression of Calcineurin, NFAT2, COX-2 and VEGF-A in four kinds of cells. Results Compared with normal melanocytes, the expressions of Calcineurin, NFAT2, COX-2 and VEGF-A4 were significantly higher in different melanoma cell lines. Conclusion Calcineurin / NFAT signaling pathway may be related to the occurrence of melanoma.