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将瓠瓜的总DNA导入西瓜,D1代获得8株变异株(株系1-8),变异率为0.32%-1.77%,性状变异较小。但株系1的D2代性状变异特别大,有22.7%的果实皮包由深绿色变成白色或白皮绿网纹,接近供体皮包;果实形状有31.0%发生变异;种子的形状和色泽有33.3%发生变异;变异株咸株期功能叶过氧化物酶同工酶酶谱与受体不同,出现了供体植株的酶带;变异株部分染色体的臂长、臂比、带型与受体相比产生了明显变异。株系2-8的D2代性状变异较小。初步认为西瓜的性状变异是供体瓠瓜DNA导入的结果。
The total DNA was introduced into watermelon melon, D1 generation of 8 strains (strains 1-8), the mutation rate was 0.32% -1.77%, minor variation in traits. However, the phenotypic variation of D2 generation in strain 1 was particularly large, with 22.7% of the fruit bags turned from dark green to white or white green, close to the donor bag; 31.0% of the fruit shape was mutated; 33.3% of the variation in shape and color occurred in the mutants. The enzyme bands of donor peroxidase isoenzyme in the mutant leaves were different from those of the recipient plants, Compared with the receptor, there was a significant variation of the receptor. Variation of D2 generation traits of strain 2-8 was minor. Initially that the watermelon trait variation is the result of the donor Cucumber DNA import.