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在pH=7.4的Tris-HCl介质中,利用荧光光谱和紫外吸收光谱法,研究了一种新型蒽环类抗癌药物柔红霉素衍生物(4’-O-(α-L-夹竹桃糖基)柔红霉素,ODNR)与小牛胸腺DNA(ctDNA)的相互作用。通过离子强度的影响、KI荧光猝灭实验和单双链ctDNA作用的比较实验,分析了ODNR与ctDNA的相互作用模式。结果表明,ODNR通过嵌插方式与ctDNA发生作用。ctDNA对ODNR的荧光有明显的猝灭作用,其机理属于静态猝灭。通过Scatchard方程求得不同温度下的结合常数和结合位点数,由热力学参数确定分子间作用力为疏水作用,也可能存在静电作用。
A new anthracycline anti-cancer drug daunorubicin derivative (4’-O- (α-L-Acridine) was studied by fluorescence spectroscopy and UV absorption spectroscopy in Tris-HCl medium at pH 7.4. Peachyl) daunorubicin, ODNR) with calf thymus DNA (ctDNA). The interaction between ODNR and ctDNA was analyzed by the influence of ionic strength, KI fluorescence quenching experiment and single-double-stranded ctDNA. The results showed that ODNR interacted with ctDNA through intercalation. ctDNA has obvious quenching effect on the fluorescence of ODNR, the mechanism belongs to static quenching. The binding constants and binding sites at different temperatures are obtained by the Scatchard equation. The interaction between molecules is determined to be hydrophobic by thermodynamic parameters, and electrostatic interaction may also exist.