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目的:了解系统性红斑狼疮(SLE)患者外周血单核细胞主要组织相容性复合体(MHC)Ⅰ类链相关分子(MⅠCs)、IL-15表达水平变化及其潜在机制。方法:收集20例SLE患者及20例健康志愿者外周血,分离单核细胞及血清,采用流式细胞术检测单核细胞表面MⅠCs及mIL-15的表达,EILIS法检测血清中干扰素-γ(INF-γ)、干扰素-α(INF-α)浓度,并对单核细胞表面MⅠCs表达水平与血清INF-γ浓度作相关性分析。进一步以SLE患者血清与健康志愿者单核细胞体外孵育,在有或无抗INF-γR存在的情况下,检测单核细胞表面MⅠCs分子的表达情况。结果:SLE患者血清IFN-γ浓度与健康对照组相比有显著性差异,其中IFN-γ在SLE患者为(429.7±145.0)pg/ml[健康对照为(73.8±18.9)pg/ml],二者有显著性差异(P<0.01);单核细胞MⅠCs及mIL-15的阳性率分别为(37.0±18.1)%和(36.0±7.3)%,而健康志愿者单核细胞MⅠCs及mIL-15的阳性率为(7.2±2.4)%和(7.6±2.5)%。SLE患者MⅠCs及mIL-15阳性率显著高于健康志愿者(P<0.01);SLE患者单核细胞MⅠCs分子表达水平与血清INF-γ浓度呈正相关;SLE患者血清中高浓度的IFN-γ可以刺激正常人单核细胞表达高水平的MⅠCs及mIL-15。结论:SLE患者血清INF-γ浓度异常升高,导致外周血单核细胞表面MⅠCs及IL-15异常高表达。
Objective: To investigate the changes of MHC class I chain-related molecules (MⅠCs) and IL-15 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus (SLE) and their underlying mechanisms. Methods: Peripheral blood samples of 20 patients with SLE and 20 healthy volunteers were collected. Monocytes and serum were collected. The expression of MⅠCs and mIL-15 on monocytes were detected by flow cytometry. The levels of interferon-γ (INF-γ), IFN-α (IFN-α), and the correlation between MⅠCs expression on monocytes and serum INF-γ concentration. The SLE patient serum was further incubated with healthy volunteers monocytes in vitro, and the expression of M ⅠCs on monocytes was detected in the presence or absence of INF-γR. Results: The serum levels of IFN-γ in patients with SLE were significantly different from those in healthy controls (429.7 ± 145.0 pg / ml for IFN-γ in SLE patients [73.8 ± 18.9 pg / ml for healthy controls] (P <0.01). The positive rates of MⅠCs and mIL-15 in monocytes were (37.0 ± 18.1)% and (36.0 ± 7.3)%, respectively. However, MⅠCs and mIL- The positive rates of 15 were (7.2 ± 2.4)% and (7.6 ± 2.5)%. The positive rates of MⅠCs and mIL-15 in SLE patients were significantly higher than those in healthy volunteers (P <0.01). The expression levels of MⅠCs in mononuclear cells of SLE patients were positively correlated with the levels of serum INF-γ. The high concentrations of IFN-γ in serum of SLE patients stimulated Normal human monocytes express high levels of M ICs and mIL-15. Conclusion: The abnormal increase of serum INF-γ in SLE patients leads to abnormal expression of MⅠCs and IL-15 on the surface of peripheral blood mononuclear cells.