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评价6-羟多巴胺(6-OHDA)损毁大鼠单侧黑质制备的偏侧帕金森病动物模型。应用6-羟多巴胺损毁SD大鼠单侧黑质制备偏侧PD鼠模型。3周后根据药物诱发试验,TH免疫组化证实模型制作成功。进一步用脑微透析技术结合HPLC-ECD在体检测PD鼠纹状体多巴胺及代谢产物含量。结果:82只大鼠中有36只阿朴吗啡(APO)诱发的旋转次数>7转/min。6-OHDA注射侧黑质DA神经末稍已绝大多数被损毁。6-OHDA损毁侧纹状体多巴胺及代谢产物明显低于健侧(P<0.05,P<0.01)。应用6-OHDA制备的偏侧PD鼠模型是PD研究的理想模型之一。
An animal model of hemiparkinsonism for the preparation of unilateral substantia nigra of 6-hydroxydopamine (6-OHDA) -deficient rats was evaluated. Application of 6-hydroxydopamine destroys unilateral substantia nigra of SD rats to prepare hemiparkinsonian model in rats. Three weeks later, according to the drug-induced test, TH immunohistochemistry confirmed that the model was successfully made. The content of dopamine and metabolites in striatum of the striatum of PD rats was detected by HPLC-ECD in brain microdialysis. RESULTS: Thirty-six apomorphine (APO) -induced spins in 82 rats> 7 rev / min. 6-OHDA injection side of the substantia nigra DA nerve endings have been the vast majority of damage. 6-OHDA lesioned striatum dopamine and metabolites were significantly lower than the contralateral (P <0.05, P <0.01). The application of 6-OHDA hemiparking PD rat model is one of the ideal model of PD.