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本文应用克隆化的凝血因子Ⅷ(下称FⅧ)基因cDNA及其旁侧的DNA片段作为探针,分析了123名中国正常人和22例血友病A患者的FⅧ基因及其旁侧的多态性位点Bcl Ⅰ/18e,Xbal/221,MspI/St14和BgI I/DX13。获得了中国人上述4个位点的RFLP资料,并应用RFLP连锁分析对2例血友病A高危妊娠进行了产前诊断。
In this paper, cloned factor Ⅷ (hereinafter referred to F Ⅷ) gene cDNA and its flanking DNA fragment as a probe, analysis of 123 Chinese normal and 22 cases of hemophilia A patients with FⅧ gene and its flanking Loci Bcl I / 18e, Xbal / 221, MspI / St14 and BgI I / DX13. The RFLP data of the above 4 loci were obtained from Chinese and the prenatal diagnosis of 2 high-risk pregnant women with hemophilia A was made by RFLP linkage analysis.