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目的：应用单细胞培养系统，对本室建成的胸腺基质细胞系ＭＴＳＣ４进行细胞克隆化并鉴定。方法：在单个细胞培养中，扩增由一个细胞长成的克隆，并使之稳定生长后，进行表面标志分子和倍增时间检查，并以其分泌的细胞因子测定其生物学特性及功能。结果：获得１４个细胞克隆，它们均表达树突状细胞抗原和ＭＨＣⅡ类分子，均无角蛋白，表明各克隆均为树突状细胞，起始的ＭＴＳＣ４也是树突状细胞系。检测了细胞倍增时间和ＩＬ６、趋化因子等细胞因子，从倍增时间和因子产生能力看，各克隆差异很大。结论：从ＭＴＳＣ４细胞系克隆出性状及功能都均一的细胞克隆来看，各克隆间的细胞特性有明显区别。
OBJECTIVE: To clone and identify the MTSC4 cell line of thymic stromal cell line established in our laboratory with single cell culture system. METHODS: In a single cell culture, clones grown from one cell were expanded and allowed to grow stably. Surface marker molecules and doubling time were examined. The biological characteristics and functions of the cloned cells were determined by their secreted cytokines. RESULTS: Fourteen cell clones were obtained, all of which expressed dendritic cell antigen and MHC class II molecules with no keratin, indicating that each clone was a dendritic cell and that the initial MTSC4 was also a dendritic cell line. Detection of cell doubling time and IL 6, chemokines and other cytokines, doubling time and factor production capacity, the clones vary widely. Conclusion: From the clone of cell clone of MTSC4 cell line with uniform traits and functions, the cell characteristics of each clone are obviously different.