对澳大利亚小麦育种材料不同范围的花药培养反应进行了测定，并按不同实验程序的实际成本表示为“培养指数”。当把花药收集在培养盘中接种时间延长到１２ｈ时，运用低剂量的γ射线辐射并不能增加这些基因型的花药培养反应序列，这与以前发现的取穗、辐射及培养盘接种在３ｈ内完成并经辐射处理增加花药培养反应的结果相反。未成熟穗感染ＦｕｓａｒｉｕｍＰｏａｅ造成了培养体的显著损失，也是禾谷类花药培养中长期存在的问题。对产生绿色后代基因型来说，其成本分布为１．４澳元至８５澳元／绿苗。 The different ranges of anther culture responses to Australian wheat breeding material were measured and expressed as “culture indices” based on the actual costs of different experimental procedures. Irradiation with low doses of γ-rays did not increase the anther culture response sequence for these genotypes when the anther collection was continued in culture plates for up to 12 h, in contrast to previously found ear picks, radiation, and plate inoculation within 3 h The result of completing and processing the anther culture reaction by radiation is the opposite. Infection of Fusarium Poa with immature spikes causes significant loss of culture and is also a chronic problem in cereal anther culture. For the generation of green offspring genotypes, the cost distribution of 1.4 Australian dollars to 85 Australian dollars / green seedlings.