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本研究用BALB/C和CBA小鼠制备了肝特异性F-抗原的抗血清,在用1/5～1/80LD_(50)剂量的CCl_4对几种动物染毒后检测发现,在注射后6～48小时内可检出血清F-抗原的释出,并与SGPT有相似的时间—反应和剂量—反应关系及肝细胞出现实质性损害的改变。而用亚致死量的氯乙醇(32mg/kg)和TNT(150mg/kg)染毒的动物,却未见F-抗原有同样的反应,提示测定血清F-抗原可能在中毒性肝损害的诊断和预后,以及检测亲肝毒物的肝毒作用性质上,具有参考价值。
In this study, BALB/c and CBA mice were used to prepare antiserum against liver-specific F-antigens and tested after injecting several animals with 1/5 to 1/80 LD_(50) dose of CCl_4. The release of serum F-antigen can be detected within 6 to 48 hours and has a similar time-response and dose-response relationship with SGPT and substantial changes in liver cell damage. However, animals infected with sub-lethal doses of chlorohydrin (32 mg/kg) and TNT (150 mg/kg) did not have the same response to F-antigen, suggesting that the determination of serum F-antigen may be useful in the diagnosis of toxic liver damage. And the prognosis, as well as the hepatotoxicity of the detection of hepatotoxicity, have a reference value.