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目的:对热性中药致虚热证模型的复制和评价进行探究。方法:使用不同剂量热性中药附子、干姜、肉桂灌胃大鼠。每天观察大鼠的活动状态、毛发光泽、爪色、二便等一般状况,每天定时测定大鼠的肛温、粪便量、尿液量、饮水量、饮食量,并于体液消耗量出现明显差异时采集血液和相关组织器官,测定血清三碘甲状腺原氨酸(T3),甲状腺素(T4),血浆环磷酸腺苷(cAMP)及环磷酸鸟苷(cGMP)含量的变化和相关脏器指数。结果:与空白组比较,各模型组大鼠饮水量、体液消耗量大多数升高,动物出现虚热证的证候;且血清中T4含量呈增高趋势,T3/T4降低。模型低剂量组和模型中剂量组的cAMP和cGMP含量与空白组比较大多数显著升高,而模型高剂量组含量则有所降低。结论:灌胃热性中药可以复制出虚热证模型,体液消耗量的增加对虚热证模型复制有一定的指导意义。
OBJECTIVE: To investigate the replication and evaluation of the heat-deficiency syndrome induced by heat-induced traditional Chinese medicine. Methods: Different doses of heat herbal aconite, ginger, cinnamon irrigation rats. The daily activities of the rats were observed, the hair luster, claw color, second and other general conditions, the daily rectal temperature of rats, stool volume, urine volume, water intake, diet, and significant differences in body fluid consumption At the same time, blood and related tissues and organs were collected to measure the changes of serum triiodothyronine (T3), thyroxine (T4), plasma cAMP and cGMP, . Results: Compared with the blank group, the water consumption and body fluid consumption increased most of the rats in each model group, and the animals showed syndromes of deficiency of heat and heat syndrome. The content of T4 in the serum increased and T3 / T4 decreased. The cAMP and cGMP contents in model low-dose group and model middle-dose group were significantly higher than those in blank group, while those in model high-dose group were decreased. CONCLUSION: Fever-heat syndrome model can be replicated by gavage with traditional Chinese medicine. The increase of body fluid consumption has certain guiding significance for the replication of the syndrome of deficiency-heat syndrome.