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用还原性烟酰胺腺嘌呤二核苷酸磷酸黄递酶，简称黄递酶（ｒｅｄｕｃｅｄｎｉｃｏｔｉｎａｍｉｄｅｄｉｎｕ－ｃｌｅｏｔｉｄｅｐｈｏｓｐｈａｔｅｄｉａｐｈｏｒａｓｅ，ＮＡＤＰＨ－ｄ）组织化学的方法，在光镜下对ＮＡＤＰＨ－ｄ反应阳性细胞进行计数和计算机图像分析测定反应产物的光密度（ｏｐｔｉｃａｌｄｅｎｓｉｔｙ，ＯＤ）值，观察一氧化氮合酶（ＮＯＳ）阳性神经元及纤维在惊厥鼠上丘分布的情况。实验动物为听源性惊厥易感性大鼠，简称惊厥鼠（Ｐ７７ＰＭＣ）和正常Ｗｉｓｔａｒ大鼠，简称正常鼠。实验结果如下：（１）在大鼠上丘第Ⅰ层ＮＯＳ阳性神经元呈密集均匀分布，在第Ⅳ层其分布也是均匀的但较稀疏，第Ⅶ层只有少量ＮＯＳ阳性神经元散在分布于中部。第Ⅰ、Ⅳ、Ⅶ层ＮＯＳ阳性神经元的大小和形态是一致的。在第Ⅰ、Ⅶ层ＮＯＳ阳性神经元呈上丘的背腹方向排列。在第Ⅳ、Ⅵ层有密集的ＮＯＳ阳性纤维。（２）上丘ＮＯＳ阳性神经元的数目以在急性发作的惊厥鼠最多，其次为非急性发作的惊厥鼠和电铃刺激的正常鼠，而在无电铃刺激的正常鼠最少。（３）ＮＯＳ阳性反应的ＯＤ值在各组大鼠间无显著性差异。本研究结果显示，上丘ＮＯＳ阳性神经成分的分布有特异性；ＮＯＳ酶的活性在惊厥鼠是增高?
NADPH-d-positive cells were counted under light microscope and computerized images were obtained by histochemistry with reduced nicotinamide adenine dinucleotide phosphate diaphorase, abbreviated as diaphorase (NADPH-d) The optical density (OD) value of the reaction product was analyzed to observe the distribution of nitric oxide synthase (NOS) positive neurons and fibers in the suprascapus of convulsion mice. Experimental animals for susceptibility to auditory convulsions in rats, referred to as seizure rats (P77PMC) and normal Wistar rats, referred to as normal mice. The experimental results are as follows: (1) NOS positive neurons in the first layer of the superior colliculus were densely and uniformly distributed, while in the fourth layer, the distribution was also uniform but sparse. Only a small amount of NOS positive neurons scattered in the middle . The size and morphology of NOS positive neurons in the first, the fourth and the seventh layers were the same. In the Ⅰ, Ⅶ NOS positive neurons were arranged in the dorsoventral direction of the superior colliculus. In Ⅳ, Ⅵ layer dense NOS-positive fibers. (2) The number of NOS-positive neurons in the superior colliculus is highest in the seizure rats with acute seizures, followed by non-acute seizure rats and bell-stimulated normal rats, and the least in the normal rats without bell stimulus. (3) There was no significant difference in OD value of NOS positive reaction in each group of rats. The results of this study showed that the distribution of NOS-positive neurons in the superior colliculus was specific and the activity of NOS enzyme was increased in convulsive mice.