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目的:分析柠檬百里香叶挥发油的主要化学成分并研究其对肝癌细胞的毒性作用。方法:采用水蒸气蒸馏法从柠檬百里香叶中提取挥发油,利用GC-MS对其化学成分进行分析。共鉴定出其中32个组分,占总出峰面积的95.44%,主要成分有冰片(28.82%)、百里香酚(14.43%)、3,7-二甲基-1,6-辛二烯-3-醇(8.26%)、1-甲基-4-(α-羟基-异丙基)环己烯(8.23%)、樟脑萜(5.1%)等。MTT法检测其对肝癌HepG2细胞的毒性作用,激光共聚焦检测对NF-κβ65表达的影响。结果:挥发油抑制肝癌HepG2细胞生长的的IC50值为0.34%浓度;NF-κβ65表达的平均荧光强度为:对照组为323.25,2-10浓度精油组为84.18,2-11浓度挥发油组为197.93,2-12浓度挥发油组261.43。结论:柠檬百里香叶挥发油对HepG2细胞有很强毒性作用,其诱导细胞凋亡机制可能与NF-κβ65表达有关。
Objective: To analyze the main chemical constituents of the essential oil of lemon thyme and to study the toxic effects on the hepatoma cells. Methods: Volatile oil was extracted from lemon thyme leaves by steam distillation. The chemical constituents were analyzed by GC-MS. A total of 32 components were identified, accounting for 95.44% of the total peak area. The main components were borneol (28.82%), thymol (14.43%), 3,7-dimethyl- 3-ol (8.26%), 1-methyl-4- (α-hydroxy-isopropyl) cyclohexene (8.23%), camphene terpene (5.1%) and so on. The cytotoxicity of HepG2 cells was detected by MTT assay and the effect of laser scanning confocal microscope on the expression of NF-κβ65. Results: The IC50 value of volatile oil for inhibiting the growth of HepG2 cells was 0.34%. The mean fluorescence intensity of NF-κβ65 expression was 323.25 in control group and 84.18 in concentrations of 2 to 10 and 197.93 in volatile oil group, 2-12 Concentration of volatile oil group 261.43. CONCLUSION: Volatile oil of lemon thyme has strong toxicity on HepG2 cells, which may be related to the mechanism of NF-κβ65.