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建立白坚木皮醇(Quebrachitol)含量的亲水作用色谱-蒸发光散射检测器(ZICHILIC-ELSD)测定方法。Merck ZIC-HILIC色谱柱(150×4.6mm,5μm);以乙腈-水(体积比为70∶30)为流动相,等度洗脱,流速为1.0mL/min;蒸发光散射检测器,蒸发温度为65℃,雾化温度为50℃,高纯氮压力9.9 MPa。结果表明,白坚木皮醇浓度与其色谱峰面积积分值呈很好的线性关系,相关系数为0.9997。检测限为0.08mg/mL;相对标准偏差为0.48%;加标回收率为100.3%。该方法快速、简便、准确,适合从复杂体系中快速检测白坚木皮醇的含量。
A method for the determination of Quebrachitol in ZICHILIC-ELSD was established. Merck ZIC-HILIC column (150 × 4.6 mm, 5 μm); isocratic elution with acetonitrile-water (70:30) as the mobile phase at a flow rate of 1.0 mL / min; evaporative light scattering detector evaporated The temperature is 65 ℃, the atomization temperature is 50 ℃, the pressure of high purity nitrogen is 9.9 MPa. The results showed that there was a good linear relationship between the alcohol concentration of Cortex Fraxinus and its integral of chromatographic peak area, the correlation coefficient was 0.9997. The detection limit was 0.08mg / mL; the relative standard deviation was 0.48%; the recovery was 100.3%. The method is rapid, simple and accurate, and is suitable for the rapid determination of white fir wood alcohol content from complex systems.