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虽然放射性示踪法(尤其是~(14)C)在生物合成途径的分析上特别有用,但为确定同位素结合的位置必需进行广泛的化学降解,这是它的严重缺点。就复杂的化合物分子来说,满意的方法应是只进行部分同位素分布的分析。以~(13)C代替~(14)C作为生物合成途径的研究引进了一种能完全确定带标记代谢物内同位素位置的技术。~(13)C是稳定的同位素,在天然有机物分子中占1.1％,目前已有若干种适于供生物合成研究用的富集~(13)C商品。
Although radioactive tracing (especially ~ (14) C) is particularly useful in the analysis of biosynthetic pathways, it is a serious disadvantage that extensive chemical degradation is necessary to determine the site of isotope incorporation. For complex compound molecules, a satisfactory method should be to perform only partial isotopic analysis. The use of ~ (13) C instead of ~ (14) C as a biosynthetic pathway has introduced a technique that completely determines the position of isotopes in labeled metabolites. ~ (13) C is a stable isotope, accounting for 1.1% of natural organic molecules. At present, there are several kinds of enriched ~ (13) C commodities suitable for biosynthesis research.