Sertoli细胞(支持细胞)是睾丸的主要基质细胞,对生精细胞的分化成熟提供微环境,其所分泌的多种物质对其周围及自身均有影响[1,2]。为了研究Sertoli细胞,须将其从睾丸组织中与其它细胞分离,并在体外培养。　　我们建立了Sertoli细胞的分离方法,采用幼龄大鼠的睾丸,用胶原酶、透 Sertoli cells (supporting cells) are the main stromal cells of the testes and provide a microenvironment for the differentiation and maturation of spermatogenic cells. The secreted substances affect both the periphery and themselves. To study Sertoli cells, they are separated from other cells in the testis and cultured in vitro. We have established a Sertoli cell separation method, using young rats testis, with collagenase, through