本文用四硫富瓦烯(TTF)作为酶与电极之间的电子传递体,通过牛血清白蛋白和戊二醛交联剂,把葡萄糖氧化酶固定在Nafion-TTF修饰石墨电极上,最后在电极修饰一层Nafion膜,制备成葡萄糖传感器。Nafion膜不仅能防止四硫富瓦烯流失,而且能把抗坏血酸、尿酸等电活性物质阻挡在电极外,防止其干扰,同时具有防污性能.通过实验表明TTF~+,TTT~(2+)都能够氧化葡萄糖氧化酶中的辅酶(FADH_2)。该传感器的线性范围为5.0×10~(-4)～1.05×10~(-2)mol/L,响应时间小于30s. In this paper, tetrathiafulvalene (TTF) was used as the electron mediator between the electrode and the electrode. Glucose oxidase was immobilized on Nafion-TTF modified graphite electrode by bovine serum albumin and glutaraldehyde crosslinking agent. Finally, Electrode modified layer of Nafion membrane, prepared glucose sensor. Nafion membrane can not only prevent the loss of tetrathiafulvalene, but also block the electroactive substances such as ascorbic acid and uric acid from the electrodes to prevent its interference and antifouling property at the same time.Experiments show that TTF ~ +, TTT ~ (2+) Can oxidize coenzyme in glucose oxidase (FADH_2). The linear range of this sensor is 5.0 × 10 -4 to 1.05 × 10 -2 mol / L, and the response time is less than 30 s.