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为探索热冲击(Heat Shock,简称HS下同)对soja亚属野生大豆(G.Soja)、半野生大豆(G.gracilis)、栽培大豆(G.max)三个不同进化类型大豆萌芽种子下胚轴酯酶同工酶的影响。本试验采用了6个不连续的骤变温度HS处理。研究结果表明:1)在45℃(2h)、40℃(1h)→45℃(2h)、40℃(2h)→26℃(2h)→45℃(2h)三种HS处理条件下,酶谱C区主谱带半野生大豆第5带、栽培大豆第6带均消失;野生大豆的酶谱C区的主谱带第6带幅明显变窄,色度明显减弱;三种大豆酶谱C区其它谱带有不同条数的消失;三种大豆酶谱C区在其主谱带上方均出现一条新谱带。2)三种大豆酶谱A区和B区谱带对所有HS反应均不敏感。3)在26℃(对照)、35℃(2h),40℃(2h)三种HS处理条件下,酶谱C区野生大豆主谱带第6带的色度从低温HS处理到高温HS处理逐渐减弱,而半野生大豆主谱带第5带则相反,栽培大豆C区主谱带第6带的变化没有明确的规律。
In order to explore the effects of Heat Shock (HS) on the seed germination of soja, G. soja, G. gracilis and G. max, Hypocotyl esterase isoenzyme effects. The experiment used six discontinuous sudden change temperature HS treatment. The results showed as follows: 1) Under the three HS treatments of 45 ℃ for 2h, 40 ℃ for 1h, 45 ℃ for 2h, 40 ℃ for 2h, 26 ℃ for 2h and 45 ℃ for 2h, The fifth band of semi-wild soybeans and the sixth band of cultivated soybeans disappeared in the main band of spectrum C, while the sixth band of the main band of Z-band of wild soybean was significantly narrowed and the chroma was obviously weakened. There were different bands disappeared in other bands in C region. A new band appeared in the C region of three soybean zymograms over the main band. 2) The three soybean zymograms A and B bands are not sensitive to all HS reactions. 3) Under the three HS treatments, 26 ℃ (control), 35 ℃ (2h) and 40 ℃ (2h), the chroma of the sixth band of Zymogram wild soybean band C was changed from low-temperature HS treatment to high-temperature HS treatment While the fifth band of the semi-wild soybeans is the opposite. There is no clear law about the change of the sixth band of the main band of the cultivated soybeans.