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应用组织学、酶组织化学和电镜技术,系统观察了黄曲霉毒素B_1(AFB_1)诱发的大鼠肝损伤。发现以汇管区周围肝细胞变性坏死为主的组织学改变、SDH和ATPase活性明显降低、ACPase活性增高等酶组化反应和肝窦扩张、窦内充填坏死肝细胞碎屑和凋落小体以及肝细胞核仁变性、核仁帽形成、核仁溶解、内浆网扩张、脂肪变性、大量自噬小体出现等超微结构损伤。笔者认为,AFB_1对肝细胞膜以及膜性结构的直接毒性作用是导致肝细胞急性变性坏死的主要因素。
Histological, enzymatic histochemical and electron microscopy techniques were used to systematically observe liver injury induced by aflatoxin B 1 (AFB 1) in rats. It was found that histological changes, declining SDH and ATPase activity, and increased ACPase activity, mainly hepatocyte degeneration and necrosis around the portal area, enzymatic histochemical reaction and hepatic sinusoidal expansion, sinusoidal filling, necrotic hepatocyte debris and litter bodies, and liver Nucleolar degeneration, formation of nucleocaps, nucleolytic dissolution, expansion of the endoplasmic reticulum, steatosis, and appearance of numerous autophagosomes. The author believes that the direct toxic effect of AFB 1 on hepatocyte membrane and membranous structure is the main factor leading to acute degeneration and necrosis of hepatocytes.