论文部分内容阅读
目的克隆人CD48基因,构建稳定表达CD48分子的基因转染细胞株。方法提取人外周血单个核细胞总RNA,通过RT-PCR克隆获得人CD48基因,将人CD48基因重组入逆转录病毒载体pEGZ-term,通过293T细胞的包装获得具有感染力的完整重组病毒载体,进而感染L929细胞,构建稳定表达人CD48分子的基因转染细胞株。结果成功克隆人CD48基因和构建PGEZ/CD48逆转录病毒表达载体,进而成功获得稳定表达人CD48的基因转染细胞株L/CD48。结论成功克隆了人CD48基因及其逆转录表达载体,并构建了稳定表达CD48分子的基因转染细胞株,为探讨CD48生物学功能的研究奠定了物质基础。
Objective To clone human CD48 gene and construct a stable cell line transfected with CD48. Methods Total RNA was extracted from human peripheral blood mononuclear cells. The human CD48 gene was cloned by RT-PCR. The human CD48 gene was recombined into the retroviral vector pEGZ-term, and the full-length infectious recombinant viral vector was obtained by packaging 293T cells. Which in turn infected L929 cells to construct a gene transfected cell line stably expressing human CD48 molecule. Results The human CD48 gene was successfully cloned and the PGEZ / CD48 retrovirus expression vector was constructed. The L48 cells transfected with human CD48 gene were successfully obtained. Conclusion The human CD48 gene and its reverse transcriptase expression vector were successfully cloned, and the gene transfection cell line stably expressing CD48 was constructed. It laid the material foundation for the study on the biological function of CD48.