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目的检测新疆维吾尔族、哈萨克族糖耐量正常人群及2型糖尿病患者粪便中奇异菌簇和产气柯林斯菌属的水平,分析奇异菌簇和产气柯林斯菌属与2型糖尿病的相关性。方法收集新疆维吾尔族、哈萨克族糖耐量正常人群及2型糖尿病患者血样和粪便样本,采用16S r DNA实时荧光定量PCR技术检测粪便样本中奇异菌簇和产气柯林斯菌属的水平;运用Pearson分析目标菌属水平与4组人群的空腹血糖(FBG)、年龄、身高、体重、体重指数(BMI)、腰围、胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDL-C)的相关性。结果 (1)与哈萨克族糖耐量正常组比较,该民族T2DM组中奇异菌簇、产气柯林斯菌属水平均降低(P=0.012,P=0.024),两民族糖耐量正常组之间奇异菌簇、产气柯林斯菌属水平差异有统计学意义(P=0.025,P=0.007);(2)奇异菌簇水平与HDL-C(r=0.281,P=0.031)呈正相关,与体重(r=-0.514,P=0.000)、BMI(r=-0.400,P=0.002)、TC(r=-0.503,P=0.000)、LDL-C(r=-0.581,P=0.000)水平呈负相关;产气柯林斯菌属水平与HDL-C(r=0.550,P=0.000)水平呈正相关,与体重(r=-0.449,P=0.000)、BMI(r=-0.432,P=0.001)水平呈负相关。结论肠道中奇异菌簇和产气柯林斯菌属,可能与与新疆维吾尔族、哈萨克族2型糖尿病患者的脂代谢紊乱密切相关。
Objective To detect the levels of Klebsiella pneumoniae and Staphylococcus aureus in feces of normal Uygur and Kazak people with type 2 diabetes in Xinjiang Uygur Autonomous Region and Xinjiang Autonomous Region, and to analyze the relationship between the genus Clostridium and genus Clostridium and type 2 diabetes. Methods The blood samples and stool samples of Uygur and Kazak people with normal glucose tolerance and type 2 diabetes mellitus were collected. The 16S r DNA real-time PCR was used to detect the levels of Strain and genus Clostridium in stool samples. Pearson analysis The level of target bacteria was positively correlated with FBG, age, height, weight, body mass index (BMI), waist circumference, cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDLC) Low density lipoprotein cholesterol (LDL-C) correlation. Results (1) Compared with Kazakh normal glucose tolerance group, the levels of S.aureus and gas-producing Clostridium in T2DM group were significantly lower (P = 0.012, P = 0.024) (P = 0.025, P = 0.007). (2) There was a positive correlation between the level of bacteria and the HDL-C (r = 0.281, P = 0.031) (R = -0.400, P = 0.002), TC (r = -0.503, P = 0.000) and LDL-C (r = -0.581, P = 0.000) (R = -0.449, P = 0.000), BMI (r = -0.432, P = 0.001), the level of HDL-C (r = 0.550, P = 0.000) Negative correlation. Conclusions The intestinal flora and genus Clostridium may be closely related to the disturbance of lipid metabolism in Uygur and Kazak type 2 diabetic patients in Xinjiang.