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[Objective] To study the effects of Toona sinensis protein on the antioxidant capacity and nonspecific immunity of mice. [Method] Crude protein was extracted from T. sinensis by ammonium sulphate precipitation, freeze drying, and dialysis. Mice were given T. sinensis protein by intraperitoneal injection for 9d in high-, middle- and low-dosages. Serums of half of mice were selected and their SOD and GSH-Px were detected by kits. Liver, spleen, and thymus were anatomized. MDA content and spleen and thymus indices were detected. The rest half mice were given starch broth at 10d by intraperitoneal injection. Then, peritoneal fluid was taken out and the phagocytic rate of macrophage was observed. [Result] Content of T. sinensis crude protein was 84.5% and the extraction rate was 5.3%. T. sinensis protein could significantly enhance the activity of SOD in mice serum (P<0.05,high dosage P<0.01). High-dosage T. sinensis protein could enhance the content of GSH-Px (P<0.05), spleen index (P<0.05,high dosage P<0.01), and phagocytic rate of macrophage (P<0.05). [Conclusion] T. sinensis protein had certain in vivo antioxidant capacity, and could promote the nonspecific immunity of mice.
[Objective] To study the effects of Toona sinensis protein on the antioxidant capacity and nonspecific immunity of mice. [Method] Crude protein was extracted from T. sinensis by ammonium sulphate precipitation, freeze drying, and dialysis. Mice were given T. sinensis protein by intraperitoneal injection for 9d in high-, middle- and low-dosages. Serums of half of mice were selected and their SOD and GSH-Px were detected by kits. Liver, spleen, and thymus were anatomized. MDA content and spleen and thymus indices were detected. The rest half mice were given starch broth at 10d by intraperitoneal injection. Then, peritoneal fluid was taken out and the phagocytic rate of macrophage was observed. [Result] Content of T. sinensis crude protein was 84.5% and the extraction rate was 5.3%. T. sinensis protein could significantly enhance the activity of SOD in mice serum (P <0.05, high dosage P <0.01). High-dosage T. sinensis protein could enhance the content of GSH-Px ), spleen index (P <0.05 , high dosage P <0.01), and phagocytic rate of macrophage (P <0.05). [Conclusion] T. sinensis protein had certain in vivo antioxidant capacity, and could promote the nonspecific immunity of mice.