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激光扫描共焦显微术和多光子显微术等新的显微成像技术可以对厚的生物样品实现光学断层成像 ,因而在生物医学诊断领域具有重要的应用前境。在Fried的一维分辨度理论的基础上 ,系统地讨论了运用共焦扫描荧光显微术在进行光学断层成像时 ,其光学断层平面分辨度与信噪比之间的定量关系 ,建立了实际显微成像系统平面测量精度的定量计算方法。所得出的结果对于选择共焦扫描显微成像系统的最佳参数及评价所设计的显微成像系统的性能具有重要的意义。
New microscopy technologies such as laser scanning confocal microscopy and multiphoton microscopy enable optical tomography of thick biological samples and therefore have important applications in the field of biomedical diagnostics. Based on Fried’s one-dimensional theory of resolution, the quantitative relationship between the resolution and the signal-to-noise ratio of optical plane in confocal scanning fluorescence microscopy was systematically discussed. Quantitative calculation method of plane measurement accuracy of microscopic imaging system. The results obtained for the selection of confocal scanning microscopy imaging system, the best parameters and evaluation of the design of the performance of the microscopic imaging system is of great significance.