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目的探讨侵入性导管内细菌生物被膜抗吞噬作用。方法于2010年7月-2011年12月收集新生儿ICU病房危重新生儿病例呼吸机导管标本32份,对导管内壁细菌分离鉴定,选择生物膜模式菌株模拟导管中液体流动状态重建细菌生物被膜,观察细菌生物被膜形态结构及吞噬细胞与细菌生物被膜的相互作用,分析导管细菌生物被膜形成与导管伴生感染的关系。结果 32例导管标本经细菌分离培养和ATB生化鉴定,显示鲍曼不动杆菌14株,铜绿假单胞菌9株,葡萄球菌6株,真菌3株;14例鉴定的鲍曼不动杆菌标本中有8株产生生物被膜菌株;患者侵入性导管容易被细菌粘附形成生物被膜,扫描电镜可用于观察细菌生物被膜形成情况,生物被膜具有明显抗吞噬作用。结论生物被膜抗吞噬作用是感染难治的重要因素。
Objective To investigate the anti-phagocytosis of bacterial biofilm in invasive duct. Methods From July 2010 to December 2011, 32 specimens of neonatal ventilator were collected from critically ill neonatal ICU wards. The bacteria were isolated and identified from the inner wall of the catheter. The biofilm model strains were selected to simulate the fluid flow in the catheter to reconstruct the bacterial biofilm. Observe the morphological structure of bacterial biofilm and the interaction between phagocytes and bacterial biofilm, and analyze the relationship between biofilm formation of ductal bacteria and associated ductal infection. Results The results showed that Acinetobacter baumannii 14 strains, Pseudomonas aeruginosa 9 strains, Staphylococcus aureus 6 strains and fungi 3 strains were found in 32 cases of ductal samples. The results of 14 strains of Acinetobacter baumannii There were 8 strains producing biofilm strains. The invasive duct of patients was easy to be adhered by bacteria to form biofilm. Scanning electron microscopy could be used to observe the formation of bacterial biofilm and the biofilm was obviously anti-phagocytosis. Conclusion The biofilm anti-phagocytosis is an important factor in refractory infection.