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以针刺造成大白鼠脑干损伤后，用ＬＳＡＢ法检测脑干组织中胶质原纤维酸性蛋白（ＧＦＡＰ）阳性细胞数目在不同损伤时间的改变。结果发现：①正常对照组，ＧＦＡＰ阳性细胞的分布在脑干的不同部位是不同的，但在同一部位较为一致。②生前损伤组，同一部位的ＧＦＡＰ阳性细胞数目随损伤时间发生改变，先增多，到达最高峰后即减少，其后又有增多趋势；但不同部位的ＧＦＡＰ阳性细胞数目增减的时间过程不尽相同。③死后损伤组，各部位的ＧＦＡＰ阳性细胞数目与正常对照组的相应部位相比无明显改变。实验说明ＧＦＡＰ阳性细胞数目的改变可区别脑干的生前、死后损伤，并可能用于推断脑干损伤时间。由于在损伤后短时间内即有改变，故可作为脑干损伤早期的诊断指标。
Acupuncture induced brain injury in rats. The number of glial fibrillary acidic protein (GFAP) positive cells in brainstem tissue was detected by LSAB method at different injury time. The results showed that: ① The normal control group, GFAP positive cells in different parts of the brain stem are different, but more consistent in the same location. (2) The number of GFAP positive cells in the same site changed with the injury time, increased first and then decreased after reaching the peak, and then increased again; however, the time course of increasing or decreasing the number of GFAP positive cells in different sites was not complete the same. ③ After injury, there was no significant change in the number of GFAP positive cells in each group compared with that in the normal control group. Experiments show that changes in the number of GFAP positive cells can distinguish between pre-and post-mortem brain stem injury, and may be used to infer the injury time of brain stem. As a result of a change in a short period of time after injury, it can be used as a diagnostic indicator of early brain stem injury.