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目的：检测化疗前后肝母细胞瘤（ＨＢ）细胞凋亡、评价其在判断ＨＢ预后及其化疗疗效中的价值并探讨化疗机制。方法：应用ＴＵＮＥＬ技术检测１３例ＨＢ化疗前后标本细胞凋亡。结果：化疗前后标本均可见ＨＢ细胞凋亡；化疗后凋亡指数（ＰＣＡＩ）较化疗前自发性凋亡指数（ＳＡＩ）显著增高（Ｐ＜００５），ＰＣＡＩ与ＳＡＩ呈正相关（ｒ＝０９０７），化疗后肿瘤体积（直径）减少值与ＳＡＩ呈正相关（ｒ＝０６９４），ＳＡＩ高者术后生存时间较ＳＡＩ低者长（Ｐ＜００５）。结论：诱导ＨＢ细胞凋亡可能是化疗药物杀死瘤细胞的重要作用机制；ＨＢ细胞自发性凋亡能力与其发生发展及化疗效果密切相关，细胞凋亡的检测可为判断预后和化疗疗效提供理论依据。
Objective: To detect the apoptosis of hepatoblastoma (HB) cells before and after chemotherapy, evaluate its value in judging the prognosis of HB and its curative effect, and to explore the mechanism of chemotherapy. METHODS: TUNEL was used to detect the apoptosis of 13 samples before and after HB chemotherapy. Results: The apoptosis of HB cells was observed before and after chemotherapy. The apoptosis index (PCAI) after chemotherapy was significantly higher than the spontaneous apoptosis index (SAI) before chemotherapy (P<005). The PCAI was positively correlated with SAI (r=0). 907), the decrease in tumor volume (diameter) after chemotherapy was positively correlated with SAI (r = 0694), and the survival time of those with higher SAI was longer than that of SAI (P < 0.05). CONCLUSION: Inducing apoptosis of HB cells may be an important mechanism for tumor cells killing by chemotherapeutic drugs; the spontaneous apoptosis of HB cells is closely related to its occurrence and development and the effect of chemotherapy. The detection of apoptosis may provide a theoretical basis for judging prognosis and chemotherapy efficacy. in accordance with.