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目的通过研究旋转刺激诱发运动病时大鼠饮食行为的变化,考察几项评价指标的运动病特异性。方法 30只SD大鼠随机分为高岭土组、糖精水组、正常饮食组、禁食组、禁水组,通过水平变速旋转刺激的方法建立大鼠运动病模型,采用自身对照,以运动病指数、24 h高岭土摄取量、24 h糖精水摄取量、24h摄食量、24 h摄水量、禁食15 h后1 h、2 h摄食量和禁水24 h后1 h、2 h摄水量作为衡量运动病反应程度的指标。结果与旋转刺激前相比,运动病指数和24 h高岭土摄取量明显增加,而24 h糖精水摄取量、24 h摄食量、24 h摄水量、禁食15 h后1 h、2 h摄食量和禁水24 h后1 h、2 h摄水量则明显减少。24 h高岭土摄取量、24 h摄水量、24 h摄食量和24 h糖精水摄取量和时间的相关系数依次为:0.998、0.982、0.969和0.697。结论大鼠运动病模型的评价宜采用几项评价指标综合判断的方法,这样有利于提高判断的准确性。其中24 h摄食量和24 h摄水量与时间的相关度较高,特异性好,值得在今后的动物研究中加以应用。
Objective To study the changes of dietary behaviors of rats induced by rotational stimulation and to investigate the motion sickness specificity of several evaluation indexes. Methods Thirty Sprague-Dawley rats were randomly divided into three groups: Kaolin group, saccharin group, normal diet group, fasting group and water-forbidden group. Rat models of motion sickness were established by horizontal variable speed rotational stimulation. , Intake of 24 h kaolin, water intake of 24 h saccharin, intake of 24 h, water intake of 24 h, food intake of 1 h and 2 h after fasting of 15 h, water intake of 1 h and 2 h after water forbidden for 24 h Indicators of the degree of motion sickness. Results Compared with pre-rotation stimulation, the motion sickness index and 24 h kaolin uptake increased significantly, while the intake of 24 h saccharin, 24 h intake, 24 h water intake, 1 h and 2 h food intake after 15 h fasting And water for 24 h after 1 h, 2 h water intake decreased significantly. The correlation coefficients of 24 h kaolin intake, 24 h water intake, 24 h food intake and 24 h saccharin water intake and time were 0.998, 0.982, 0.969 and 0.697, respectively. Conclusion The evaluation of rat model of motion sickness should adopt several methods of comprehensive evaluation of evaluation indexes, which will help to improve the accuracy of judgment. Among them, 24 h food intake and 24 h water intake had higher correlation with time and specificity, which is worth to be applied in animal studies in the future.