论文部分内容阅读
目的探讨系统性红斑狼疮(SLE)患者骨髓间质干细胞(MSCs)生物学特性、染色体核型异常与否及其对造血支持的影响。方法采用密度梯度离心和贴壁分离法对11例SLE患者和6名正常人骨髓MSCs进行分离培养,流式细胞术鉴定MSCs表面标志物,观察MSCs形态学改变及生长状况并绘制生长曲线,秋水仙素终止法分析MSCs染色体核型,正常人和SLE患者的MSCs输注大剂量化疗处理的ICR小鼠,观察正常人和SLE患者MSCs对小鼠造血的影响。结果SLE患者MSCs前5代可共收获(6～9)×10~9个细胞,其生长较正常人缓慢(P＜0．01),均表达CD29、CD44和CD105,均不表达CD14、CD34、CD45和HLA-DR,染色体核型正常,正常人和SLE患者MSCs输注大剂量化疗处理的ICR小鼠后,小鼠白细胞、血红蛋白和血小板恢复均较未输注MSCs小鼠快(P＜0．05)。结论SLE患者MSCs体外生长存在一定缺陷,染色体核型正常,有支持造血功能。
Objective To investigate the biological characteristics of bone marrow mesenchymal stem cells (MSCs) in patients with systemic lupus erythematosus (SLE), the abnormality of karyotypes and their effects on hematopoietic support. Methods MSCs of 11 SLE patients and 6 normal individuals were isolated and cultured by density gradient centrifugation and adherent separation. MSCs surface markers were identified by flow cytometry. Morphological changes and growth status of MSCs were observed and the growth curve was drawn. Autumn Narcissus termination method analysis MSCs chromosome karyotype, normal and SLE patients with MSCs infusion of high-dose chemotherapy ICR mice were treated to observe the effects of MSCs on hematopoiesis in normal and SLE patients. Results The first 5 generations of MSCs in SLE patients were able to harvest a total of 6 ~ 9 × 10 ~ 9 cells. The growth of MSCs was slower than that of normal individuals (P <0.01), and both of them expressed CD29, CD44 and CD105, , CD45 and HLA-DR, and normal karyotype. The recovery of white blood cells, hemoglobin and platelet in mice treated with high-dose chemotherapy after MSCs were infused into normal and SLE patients were faster than those without MSCs (P < 0.05). Conclusion There are some defects in the growth of MSCs in vitro in SLE patients. The chromosomes of karyotype are normal and support hematopoietic function.