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目的:建立大鼠1型糖尿病模型,探讨糖尿病早期心脏抗氧化能力及心功能改变与氧自由基所致脂质过氧化反应的关系。方法:Wistar鼠16只,体重(250±10)g,随机分为对照组(n=8)及糖尿病组(n=8)。糖尿病模型通过尾静脉注射链脲佐菌素(60 mg/kg)制备。成功制备糖尿病模型后4周观察心脏功能、心肌总抗氧化能力及脂质过氧化产物15-F2t-Isoprostane变化及血清总抗氧化浓度。结果:与对照组相比,4周糖尿病鼠血清及心脏15-F2t-Isoprostane浓度无差异,但糖尿病鼠心脏组织在抗氧化剂t-butylhydroperoxide(t-BHP)刺激下,硫代巴比妥酸反应物质(TBARS)产物显著增强,反映心脏抗氧化能力降低,同时伴有心脏射血分数的显著下降(P<0.05)。结论:糖尿病鼠早期心脏抗氧化能力及心功能的减退早于脂质过氧化损伤的增加。
OBJECTIVE: To establish a rat model of type 1 diabetes mellitus to investigate the relationship between cardiac antioxidant capacity and cardiac function in early stage of diabetes and lipid peroxidation induced by oxygen free radicals. Methods: Sixteen Wistar rats weighing 250 ± 10 g were randomly divided into control group (n = 8) and diabetic group (n = 8). Diabetic models were prepared by injecting streptozotocin (60 mg / kg) through the tail vein. Four weeks after the successful preparation of diabetic model, cardiac function, total myocardial antioxidant capacity and changes of 15-F2t-Isoprostane and total serum anti-oxidant concentrations were observed. Results: Compared with the control group, there was no difference in 15-F2t-Isoprostane concentrations in the serum and heart of the 4-week diabetic rats. However, the cardiac tissue of diabetic rats were stimulated by the t-butylhydroperoxide (t-BHP) Substance (TBARS) product was significantly increased, reflecting the reduced ability of anti-oxidative heart, accompanied by a significant decline in cardiac ejection fraction (P <0.05). Conclusion: The anti-oxidative capacity and cardiac function of early diabetic mice were earlier than that of lipid peroxidation.