已有的研究表明:EGFR的过量表达增强了肿瘤的转移,但其确切的机理还知之甚少.同时,肿瘤细胞对胞外基质的粘附是肿瘤侵袭转移的重要步骤.为了探讨EGFR的表达改变是否影响肿瘤细胞对胞外基质的粘附作用,我们将构建的EGh反义序列表达质粒(pLXSN/AS)导入人过量表达EGFR的人类鼻咽癌细胞侏CNE-2Z中(pLXSN/AS)作对照转染).经G418筛选及125I-EGF结合分析,获得EGFR下调表达的CNE-2Z/AS克隆细胞,继而分别将CNE-2Z/AS,CNE-2Z及CNE-2Z/pLXSN细胞铺入含有层粘连蛋白(LN,10mg/ml)或纤粘连蛋白(FN,10mg/ml)的平皿中,于培养20min,40min,60min分别计数粘附于平的细胞数并绘制细胞生长曲线.结果表明:EGFR的下调表达能明显降低CNE-2Z细胞对FN,LN的粘附作用,而对照组细胞对FN,LN的粘附作用没有改变.这一结果提示:肿瘤细胞中EGFR的过量表达增强了肿瘤细胞对胞外基质的粘附作用,从而促进了肿瘤细胞分解基底膜和远端转移,这为进一步研究肿瘤转移机制奠 Previous studies have shown that: EGFR overexpression enhances tumor metastasis, but its exact mechanism is poorly understood.At the same time, adhesion of tumor cells to extracellular matrix is ​​an important step in tumor invasion and metastasis.In order to explore the expression of EGFR (PLXSN / AS) in human nasopharyngeal carcinoma cells overexpressing EGFR were transfected with the constructed expression vector pEGFP-EGFP antisense plasmid (pLXSN / AS) As a control, transfected with CNE-2Z / AS, CNE-2Z and CNE-2Z / pLXSN cells were screened by G418 and 125I-EGF binding analysis Plates containing laminin (LN, 10 mg / ml) or fibronectin (FN, 10 mg / ml) were counted in flat cells and incubated for 20 min, 40 min and 60 min, respectively, and the cell growth curve was plotted. : The downregulation of EGFR could significantly reduce the adhesion of CNE-2Z cells to FN and LN, while the adhesion of the control group to FN and LN did not change.This result suggested that the overexpression of EGFR in tumor cells increased Tumor cells adhere to the extracellular matrix and promote tumor cell decomposition Transfer membrane and a distal end, which is a further study of mechanism of tumor metastasis Dien