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绿色荧光蛋白 (ｇｒｅｅｎｆｌｕｏｒｅｓｃｅｎｔｐｒｏｔｅｉｎ ,ＧＦＰ)来源于可自发荧光的海洋水母 ,其ｃＤＮＡ所编码的蛋白质受到蓝光或紫外光照射时可自发的发出绿色荧光。我们将ＧＦＰ质粒ＤＮＡ转染不同类型的细胞 ,利用ＧＦＰ蛋白标记活细胞。一、方法我们采用的ＧＦ
The green fluorescent protein (GFP) is derived from an autofluorescent marine jellyfish whose cDNA encodes a protein that spontaneously emits green fluorescence when exposed to blue or ultraviolet light. We transfected different types of cells with GFP plasmid DNA and labeled living cells with the GFP protein. First, the method we use GF