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目的通过比较罗格列酮和辛伐他汀对动脉粥样硬化兔炎症因子的影响,探讨其抗动脉粥样硬化的作用机制。方法 36只雄性新西兰大白兔随机分为对照组、模型组、辛伐他汀组、罗格列酮组,采用皮下注射同型半胱氨酸硫内酯及高脂饮食相结合的方法建立动脉粥样硬化模型,酶联免疫吸附法检测血清白介素-18水平,免疫组化技术测定主动脉粥样斑块中单核细胞趋化蛋白-1和基质金属蛋白酶-9的表达。结果与对照组比较,其他3组血清白介素-18水平、主动脉粥样斑块中单核细胞趋化蛋白-1和基质金属蛋白酶-9表达显著增高(P<0.05);罗格列酮组和辛伐他汀组白介素-18水平、单核细胞趋化蛋白-1和基质金属蛋白酶-9表达较模型组明显降低(P<0.05)。罗格列酮组白介素-18较辛伐他汀组降低明显(P<0.05),单核细胞趋化蛋白-1和基质金属蛋白酶-9的表达在两组间无统计学差异(P>0.05)。结论罗格列酮可降低动脉粥样硬化兔血清IL-18和主动脉粥样斑块中单核细胞趋化蛋白-1和基质金属蛋白酶-9的表达,抑制动脉粥样硬化的进展,其机制与抗炎作用有关。
Objective To compare the effects of rosiglitazone and simvastatin on inflammatory cytokines in atherosclerotic rabbits and to explore the mechanism of anti-atherosclerosis. Methods Thirty-six male New Zealand white rabbits were randomly divided into control group, model group, simvastatin group and rosiglitazone group. Atherosclerosis was induced by subcutaneous injection of homocysteine and high-fat diet Sclerosis model, serum interleukin-18 level by enzyme-linked immunosorbent assay, monocyte chemoattractant protein-1 and matrix metalloproteinase-9 in aortic atherosclerotic plaque were detected by immunohistochemistry. Results Compared with the control group, the levels of serum interleukin-18, monocyte chemoattractant protein-1 and matrix metalloproteinase-9 in aortic atherosclerotic plaques were significantly increased in the other three groups (P <0.05); rosiglitazone group Compared with model group, the levels of IL-18, MCP-1 and MMP-9 in simvastatin group were significantly lower than those in model group (P <0.05). Interleukin-18 in rosiglitazone group was significantly lower than that in simvastatin group (P <0.05). The expression of MCP-1 and MMP-9 were not significantly different between the two groups (P> 0.05) . Conclusions Rosiglitazone can reduce the expression of monocyte chemoattractant protein-1 and matrix metalloproteinase-9 in the serum of atherosclerotic rabbits IL-18 and aortic atherosclerotic plaque and inhibit the progression of atherosclerosis Mechanism and anti-inflammatory effect.