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目的检测卵巢癌细胞的外排小体Hs-exosomes致敏树突状细胞DC诱导T淋巴细胞CTL特异性杀伤卵巢癌细胞的能力。方法:分离卵巢癌细胞株2780释放的Hs-exosomes,将其致敏DC并与T淋巴细胞共培养,检测CTL体外细胞毒活性。结果:Hs-exosomes致敏DC激活CTL的抗肿瘤能力显著高于未致敏DC组。结论:2780细胞释放的Hs-exosomes致敏DC激活T淋巴细胞对肿瘤具有很强的杀伤作用。
Objective To detect the ability of CTL-specific killing of ovarian cancer cells by DCs induced by dendritic cells (DCs) excreted by Hs-exosomes in ovarian cancer cells. Methods: The Hs-exosomes released from ovarian cancer cell line 2780 were isolated and their DCs were primed and co-cultured with T lymphocytes to detect the cytotoxic activity of CTL in vitro. Results: The antitumor activity of CTL activated by Hs-exosomes sensitized DCs was significantly higher than that of non-sensitized DCs. Conclusion: Hs-exosomes released from 2780 cells sensitize DCs to activate T lymphocytes and have a strong killing effect on the tumor.