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目的:建立高效液相色谱法测定颠胃酸口服液中东莨菪碱含量的方法。方法:色谱柱为Diamonsil-C18柱(250 mm×4.6 mm,5μm),流动相为0.25%十二烷基硫酸钠溶液(用磷酸调pH为2.5)-乙腈(60∶40),流速1.0 mL/min,检测波长210 nm,进样量20μL,柱温30℃。结果:东莨菪碱在2.6～130.0μg/mL范围内线性关系良好,A=0.3075C-0.3101(r=0.9995),回收率为97.3%(RSD=0.93%,n=6)。结论:所建立的方法简便快捷,专属性强,重现性好,适用于颠胃酸口服液中东莨菪碱的含量测定。
Objective: To establish a HPLC method for the determination of scopolamine in the stomach acid oral solution. METHODS: Diamonsil-C18 column (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of 0.25% sodium dodecyl sulfate (adjusted to pH 2.5 with phosphoric acid) / min, detection wavelength 210 nm, injection volume 20μL, column temperature 30 ℃. Results: Scopolamine showed a good linearity in the range of 2.6-130.0 μg / mL with A = 0.3075C-0.3101 (r = 0.9995). The recovery rate was 97.3% (RSD = 0.93%, n = 6). Conclusion: The established method is simple, rapid, specific and reproducible, and is suitable for the determination of scopolamine in the stomach acid oral liquid.