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目的 研究生长抑素对人红白血病K5 6 2细胞的抑制作用。 方法 采用 8肽生长抑素———奥曲肽(octreotide ,SMSZOL 1995 ,SMS)在体外作用于K5 6 2细胞 ,经光镜、电镜观察、3H TdR掺入法和TdT缺口末端标记(TUNEL)技术检测K5 6 2细胞增殖、凋亡的变化。 结果 光镜下观察到 ,加SMS组培养 72h与空白对照组比较 ,细胞碎片增多 ;电镜下可见 ,SMS处理组中部分细胞核染色质靠核膜边集 ,呈块状或新月形 ,部分细胞胞浆中见核碎裂团块与空泡 ,线粒体基质深染、空泡样变和髓样变 ;3H TdR掺入法显示 ,SMS呈浓度依赖性地抑制K5 6 2细胞增殖 ,抑制范围为 2 0 %～ 5 0 % ,以 10 - 6 mol L最有效。在 10 - 1 0 ～ 10 - 6 mol L范围内 ,与对照组比较有显著性差异 (P <0 0 1) ;TUNEL检测见核深染的阳性细胞 ,10 - 6 mol L组与对照组比较 ,凋亡细胞明显增多 (P <0 0 1)。 结论 生长抑素可抑制K5 6 2细胞增殖 ,诱导凋亡。
Objective To study the inhibitory effect of somatostatin on human erythroleukemia K5 6 2 cells. Methods K562 cells were treated with octreotide (SMSZOL 1995, SMS) in vitro. The cell viability was measured by light microscopy, electron microscopy, 3H TdR incorporation and TdT nick end labeling (TUNEL) K5 6 2 cell proliferation and apoptosis. Results In the light microscope, the cell debris was increased in the SMS group compared with the blank control group at 72h. The electron microscopy showed that part of the nuclear chromatin in the SMS-treated group was clustered by nuclei and showed clumps or crescent shape. Some cells In the cytoplasm, nucleus fragmentation and vacuolization were observed. The mitochondria stromal cells were deeply stained, vacuolar degeneration and myeloid degeneration. 3H-TdR incorporation showed that SMS inhibited the proliferation of K562 cells in a concentration-dependent manner with inhibition range of 20% ~ 50%, with 10 - 6 mol L most effective. There was a significant difference (P <0.01) in the range of 10 - 10 ~ 10 - 6 mol L compared with that of the control group. Nuclear staining cells were detected by TUNEL assay. Compared with the control group, 10 - 6 mol L group , Apoptotic cells increased significantly (P <0.01). Conclusion Somatostatin can inhibit K562 cell proliferation and induce apoptosis.