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目的:采用基因芯片技术,分别构建气虚血瘀证大鼠和红花注射液给药处理后气虚血瘀证大鼠的差异基因表达谱,比较并分析,筛选出红花能够治疗气虚血瘀证的关键基因群,并推测其起治疗作用的基因组调控机制。方法:15只SD大鼠随机分为模型组、给药组、空白对照组。模型组和给药组采用疲劳游泳和饥饿饲养处理。造模一周后,给药组尾静脉注射红花注射液(100mg/kg/d),模型组给予相同体积生理盐水;对照组不做任何处理。造模进行两周后处死大鼠,取血检验血流变指标并评价造模情况;另抽取足够的血分离mRNA并逆转录杂交基因芯片;扫描信号分析确定受红花注射液调控的基因;并通过基因数据库查询相关基因功能,结合相关文献分析初步探讨红花作用的机制。结果:两周后经过检验和观察发现模型组大鼠在不同切率下的全血粘度增加,并且其体征表现出虚弱和瘀血的状态、体重下降,确定造模成功;给药组大鼠则相对于模型组的各项检测指标和状态有所改善,确认药物有疗效。在差异基因的比较中,空白组相对于给药组上调基因252条,下调基因54条;给药组相对于模型组上调基因196条,下调基因32条;两次差异表达基因中有16条相同基因,这些差异基因涉及到炎症损伤、免疫调节反应等方面。结论:红花注射液对于气虚血瘀证有治疗作用,在基因层次上是通过抗炎症损伤机制实现的。
OBJECTIVE: To construct differential gene expression profiles of Qi-deficiency and blood-stasis rats after Qi-deficiency and blood-stasis syndrome rats and Honghua injection administration by gene chip technology. The results showed that safflower could treat Qi deficiency and blood stasis syndrome Of the key gene group, and speculated that it plays a therapeutic role in the genomic regulatory mechanism. Methods: Fifteen SD rats were randomly divided into model group, administration group and blank control group. The model group and the administration group were treated with fatigue swimming and starvation feeding. One week after modeling, safflower injection (100mg / kg / d) was given to the tail vein of the administration group. The same volume of normal saline was given to the model group. The control group was given no treatment. Rats were sacrificed two weeks after the model was established, blood samples were collected to assess the blood rheology and to evaluate the modeling conditions. In addition, enough blood was collected to isolate mRNA and cDNA was reverse transcribed. Genes regulated by safflower injection were identified by scanning signal analysis. And through gene database query related gene function, combined with relevant literature analysis of the mechanism of safflower effect. Results: After two weeks, the whole blood viscosity of rats in model group increased at different cut-off rates and the signs and symptoms of the model group showed weakness and blood stasis, Then relative to the model group of the test indicators and status have improved, to confirm the efficacy of drugs. In the comparison of the differential genes, 252 genes were upregulated in the blank group and 54 genes were down-regulated in the blank group; in the drug-treated group, 196 genes were up-regulated and 32 genes were down-regulated in the control group; 16 of the differentially expressed genes The same genes, these differences in genes involved in inflammation, immune response and so on. Conclusion: Safflower injection has a therapeutic effect on qi deficiency and blood stasis syndrome, and is at the genetic level through anti-inflammatory injury mechanism.