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bZIP蛋白是植物转录因子中最大和最保守的一类转录因子,参与调控植物生长发育等多种生命活动。谷子(Setaria italica)是一种重要的C4杂粮作物,其b ZIP基因家族与功能报道较少。利用生物信息学工具,从谷子全基因组中鉴定出73个Sib ZIP转录因子,划分为A、B、C、D、E、G、H、I和X等亚家族。与已测序的禾谷类作物相比,谷子Sib ZIP基因家族在进化中发生缩减。在谷子Sib ZIP蛋白中检测到25种不同的保守氨基酸基序。RNA-seq和定量PCR检测结果表明,在干旱和盐胁迫条件下,多数Sib ZIPs基因不同程度地被诱导表达,预示着部分Sib ZIP成员在谷子干旱和盐胁迫响应中起重要作用。共表达关联性分析进一步揭示19个谷子Sib ZIP转录因子可通过与蛋白激酶或NPR1相关调节蛋白等互作介导谷子胁迫响应。研究结果为全面解析谷子Sib ZIPs基因结构与生物学功能、抗旱分子机制以及分子育种提供了新信息。
BZIP protein is the largest and most conservative transcription factor in plant transcription factors, which is involved in the regulation of various life activities such as plant growth and development. Setaria italica is an important C4 grain crop with few reports on the b ZIP gene family and function. Using bioinformatics tools, 73 Sib ZIP transcription factors were identified from the whole genome of millet and divided into subfamilies A, B, C, D, E, G, H, I, Compared with the sequenced cereal crops, the millet Sib ZIP gene family is evolved to diminish. Twenty-five different conserved amino acid motifs were detected in the millet Sib ZIP protein. The results of RNA-seq and quantitative PCR showed that most Sib ZIPs genes were induced to some extent under drought and salt stress, indicating that some Sib ZIP members play an important role in millet drought and salt stress response. Co-expression correlation analysis further revealed that 19 alleles of Sib ZIP transcription factor could mediate response to millet stress through interaction with protein kinase or NPR1-related regulatory proteins. The results provide new information for comprehensive analysis of the structure and biological function of Sib ZIPs gene in rice, molecular mechanism of drought resistance and molecular breeding.