本文研究了二苯碳酰二肼(DPC)—溴化十六烷基吡啶(CPB)—铜超高灵敏显色反应和吸光光度法测定微量铜的测定条件。络合物最大吸收波长510nm,表观摩尔吸光系数ε=1.65×10~6,铜量在0.05～1.0μg/25ml范围内服从比耳定律,该法灵敏度极高,选择性良好,并将此法应用于水、食品中微量铜测定,结果满意。 In this paper, the conditions for the determination of trace copper with ultra sensitive color reaction of dibenzohydrazine (DPC) - cetyl pyridinium bromide (CPB) - copper and spectrophotometry were studied. The maximum absorption wavelength of the complex is 510nm, the apparent molar absorptivity ε = 1.65 × 10 ~ 6, the Beer’s law is obeyed in the range of 0.05-1.0μg / 25ml. This method has high sensitivity and good selectivity. Law applied to water, trace copper determination in food, the results are satisfactory.