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目的探讨骆驼乳对四氯化碳致小鼠肝损伤的保护作用。方法取健康成年雄性SPF级小鼠50只,体重为18～22 g,随机分为空白对照组、模型对照组及骆驼乳低、中、高剂量组,分别为2.5、5、15g/(kg.d),每组10只,空白对照组和模型对照组动物灌胃给予等量蒸馏水。实验第30天将动物隔夜禁食16 h后,模型对照组及驼乳组动物一次灌胃1%的四氯化碳油溶液0.05 ml/10 g,空白对照组灌胃同体积植物油,受试组继续给予受试样品至实验结束(与四氯化碳灌胃间隔4 h以上);24 h后处死动物,取血测定血清中的丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的含量;取动物肝脏进行肝脏病理组织学检测。结果与对照组相比,CCl4模型组血清ALT和AST活力明显升高,病理评分明显高于对照组(P<0.01)。与模型组相比,骆驼乳低、中和高剂量组血清ALT、AST活力明显降低(P<0.01);同时骆驼乳可有效减轻CCl4引起的细胞坏死、气球样变等肝细胞损伤(P<0.01)。结论骆驼乳对四氯化碳引起的小鼠肝脏病理损伤具有一定的保护作用。
Objective To investigate the protective effect of camel milk on liver injury induced by carbon tetrachloride in mice. Methods Fifty healthy adult male SPF mice weighing 18-22 g were randomly divided into blank control group, model control group and low, medium and high dose group of camel milk, which were 2.5, 5 and 15 g / (kg .d), each group of 10, blank control group and model control group animals were given equal volume of distilled water. Animals were fasted overnight for 16 h on the 30th day of the experiment. The model control group and the camelid group were orally given 1% of the CCl 4 oil solution 0.05 ml / 10 g, while the blank control group was given the same volume of vegetable oil, Group continued to give the test sample to the end of the experiment (intragastric administration of carbon tetrachloride and more than 4 h); animals were sacrificed after 24 h, blood was taken for determination of alanine aminotransferase (ALT) and aspartate aminotransferase AST); take animal liver for histopathological examination. Results Compared with the control group, the activity of ALT and AST in serum of CCl4 model group was significantly increased, and the pathological score was significantly higher than that of the control group (P <0.01). Compared with the model group, the activities of ALT and AST in the low, middle and high dose of camel milk significantly decreased (P <0.01), while the camel milk could effectively reduce the hepatocellular injury induced by CCl4 (P < 0.01). Conclusion Camel milk has a protective effect on liver injury induced by carbon tetrachloride in mice.