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目的探讨丙二醛(malondialdehyde,MDA)和腺苷A1受体(adenosine A1 receptor,A1R)的表达差异与幼年和成年大鼠皮层注射FeCl3所致痫性发作的相关性及其在大鼠痫性发作中的可能机制。方法 12只成年大鼠分为对照组和模型组,分别皮层注射生理盐水和FeCl3;18只幼鼠分为3组:对照组和模型组处理同对应成年大鼠;干预组大鼠皮层注射FeCl3前30 min行腹腔注射A1R拮抗剂(DPCPX)处理。大鼠被严密观察6 h后处死,用硫代巴比妥酸(TBA)法检测脑脊液中MDA含量,RT-PCR、Western blot检测注射皮层A1R表达。结果模型组、干预组大鼠出现典型的痫性发作;幼鼠模型组较成年鼠模型组癫痫发作次数明显增多[(12.70±7.63)次vs(3.20±2.37)次,P<0.01];干预组[(13.10±7.71)次]与幼鼠模型组之间典型癫痫发作次数无统计学差异(P>0.05);幼鼠模型组癫痫发作程度较重,持续时间较长,与成年鼠模型组比较,差异显著[(10.17±6.73)min vs(2.43±1.87)min,P<0.01];干预组大鼠癫痫发作程度最重,持续时间最长(14.25±5.82)min,与幼鼠模型组比较,差异显著(P<0.05)。与生理盐水对照组比较,模型组注射区域脑皮层A1R mRNA表达下降(P<0.05),且幼鼠较成年鼠表达下降趋势更为明显(P<0.05);干预组注射区域脑皮层A1R mRNA表达与幼鼠模型组比较,下降更为显著(P<0.05)。A1R蛋白表达趋势与RT-PCR检测结果一致。与生理盐水对照组比较,模型组大鼠脑脊液中MDA表达升高(P<0.05),且幼鼠较成年鼠表达升高趋势更为明显(P<0.05);干预组幼鼠脑脊液中MDA表达与幼鼠模型组比较,升高最为显著(P<0.05)。结论 MDA升高和A1R下降程度不同,可能是幼年和成年大鼠不同痫性发作的原因之一。
Objective To investigate the correlation between the expression of malondialdehyde (MDA) and adenosine A1 receptor (A1R) and epileptic seizures induced by FeCl3 injection into the cortex of young and adult rats, Possible mechanism of attack. Methods Twelve adult rats were randomly divided into control group and model group. Normal saline and FeCl3 were injected into the cortex respectively. Eighteen young rats were divided into three groups: the control group and the model group were treated with the same adult rats; the rats in the intervention group were injected with FeCl3 The first 30 min by intraperitoneal injection of A1R antagonist (DPCPX) treatment. The rats were sacrificed 6 hours after the operation, and the contents of MDA in cerebrospinal fluid were detected by TBA method. The expression of A1R in the injected cortex was detected by RT-PCR and Western blot. Results The number of seizures in the model group and the intervention group was significantly higher than that in the adult mouse model group (12.70 ± 7.63 vs 3.20 ± 2.37, P <0.01) There was no significant difference in the number of typical epileptic seizures between the group [(13.10 ± 7.71)] and the young mouse model group (P> 0.05). The seizure severity was longer in the young mouse model group than in the adult mouse model group (10.17 ± 6.73) min vs (2.43 ± 1.87) min, P <0.01]. The rats in the intervention group had the highest seizure duration and the longest duration (14.25 ± 5.82) min, The difference was significant (P <0.05). Compared with the saline control group, the expression of A1R mRNA in the cortex of the model group decreased (P <0.05), and the expression of A1R mRNA in the cortex of the model group decreased more significantly than that of the adult mouse (P <0.05) Compared with the young mouse model group, the decline was more significant (P <0.05). A1R protein expression trends and RT-PCR test results. Compared with saline control group, the expression of MDA in cerebrospinal fluid of rats in model group increased (P <0.05), and the expression of MDA in cerebrospinal fluid of young rats increased more obviously than that in saline control group (P <0.05) Compared with the young rat model group, the increase was the most significant (P <0.05). Conclusions The elevation of MDA and the decrease of A1R may be one of the causes of different epileptic seizures in young and adult rats.