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白细胞介素－１β（ＩＬ－１β）对胰岛β细胞具有细胞毒效应。本实验采用离体培养的新生大鼠胰岛，观察ＩＬ－１β对胰岛素分泌、ＤＮＡ合成和一氧化氮（ＮＯ）生成的影响及氨基胍对胰岛功能的保护作用。结果：①ＩＬ－１β（１０、２０、４０Ｕ／ｍｌ）孵育胰岛２４小时后，可抑制急性高浓度葡萄糖刺激的胰岛素分泌和胰岛３Ｈ－ＴｄＲ掺入量，具有明显的剂量依赖性，且其抑制程度与亚硝酸盐的生成量密切相关。②氨基胍（０．１、０．２、０．４ｍｍｏｌ／Ｌ）抑制亚硝酸盐的生成，同时减轻或完全阻断ＩＬ－１β对胰岛素分泌和３Ｈ－ＴｄＲ掺入的影响，呈剂量依赖性。实验结果表明：ＩＬ－１β对胰岛β细胞的细胞毒效应由ＮＯ介导，氨基胍通过抑制ＮＯ的合成阻断了ＩＬ－１β的胰岛损伤效应。
Interleukin-1β (IL-1β) has a cytotoxic effect on pancreatic β-cells. In this study, cultured neonatal rat islets were used to observe the effect of IL-1β on insulin secretion, DNA synthesis and nitric oxide (NO) production and the protective effect of aminoguanidine on islet function. Results: ① After islet incubated with IL-1β (10, 20, 40U / ml) for 24 hours, the inhibitory effect of acute high glucose on insulin secretion and islet 3H-TdR incorporation was significantly dose-dependent And nitrite generation is closely related. ② Aminoguanidine (0.1,0.2,0.4 mmol / L) inhibited the formation of nitrite, and at the same time reduced or completely blocked the effect of IL-1β on insulin secretion and 3H-TdR incorporation in a dose-dependent manner . The experimental results showed that the cytotoxic effect of IL-1β on islet β cells was mediated by NO, and aminoguanidine blocked the effect of IL-1β on pancreatic islet lesion by inhibiting the synthesis of NO.