药物代谢研究中的一项重要任务是测定分离代谢产物的结构,这是一较为复杂的过程(图一)。分离、纯化后的代谢产物,可以通过薄层层析、红外、紫外、质谱、核磁等理化手段来鉴定。对于极少量的代谢产物,质谱是一项很重要的技术,它能够提供代谢物的分子量、各元素组成以及裂解途径,以获得进一步的结构信息。然而,一旦分离到足够量的化合物,就可从核磁共振(NMR)获得详尽的结构信息,因为NMR An important task in the study of drug metabolism is to determine the structure of the isolated metabolites, which is a more complicated process (Figure 1). Isolated, purified metabolites, can be identified by TLC, IR, UV, MS, NMR and other physical and chemical means to identify. For very small amounts of metabolites, mass spectrometry is an important technique that provides the molecular weight of the metabolites, the elemental composition and the lysis pathways for further structural information. However, once a sufficient amount of compound has been isolated, detailed structural information can be obtained from nuclear magnetic resonance (NMR) because NMR