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目的利用cDNA芯片和荧光定量PCR(Fluorescence Quantitive Polymerase Chain Reaction,FQ- PCR)技术检测难治性癫痫患者脑组织中HSPA12A(heat shock 70kDa protein 12A,HSPA12A)mRNA的表达,并探讨其在难治性癫痫发病机制中的作用。方法收集36例难治性癫痫患者术后脑组织和8例正常对照组,用含有4096条人类靶基因的cDNA芯片进行扫描,筛选出候选基因后,用FQ-PCR,以3-磷酸甘油醛脱氢酶(GAPDH)为内参对芯片扫描结果进行验证。结果发现候选基因HSPA12A mRNA在难治性癫痫患者脑组织中表达上调,FQ- PCR与cDNA芯片结果一致。结论虽然HSPA12A具体生物功能尚不明确,但我们认为HSPA12A可能作为重要的调节因子参与了神经元凋亡等分子事件。HSPA12A mRNA表达上调可能在难治性癫痫发病机制中起一定作用。
Objective To detect the mRNA expression of heat shock 70 kDa protein 12A (HSPA12A) in brain tissue of refractory epilepsy patients by cDNA microarray and Fluorescence Quantitative Polymerase Chain Reaction (FQ-PCR) The role of epilepsy in the pathogenesis. Methods Totally 36 brain tissues from patients with intractable epilepsy and 8 normal controls were collected and scanned with cDNA microarray containing 4096 human target genes. Candidate genes were screened by FQ-PCR and glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) as internal control chip scan results were verified. The results showed that the candidate gene HSPA12A mRNA expression in brain tissue of patients with refractory epilepsy, FQ-PCR and cDNA chip results. Conclusion Although the specific biological function of HSPA12A is not clear, we think that HSPA12A may play an important regulatory role in neuronal apoptosis and other molecular events. HSPA12A mRNA expression may play a role in the pathogenesis of refractory epilepsy.