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为获得刺五加FPS的DNA和启动子序列,以及其功能元件信息和基因结构,根据已克隆的FPS cDNA序列设计引物,使用TAIL-PCR技术克隆FPS的基因组DNA及启动子序列,再通过各生物软件分析基因结构与启动子功能元件。得到刺五加FPS基因全长为7 952 bp,含有12个外显子和11个内含子,启动子序列含有25个类型功能元件,包括:62个TATA-box、24个CAAT-box,以及其他类型的重要功能元件34个。本研究首次克隆得到刺五加FPS的DNA序列和启动子全长,获取了内外显子的分布情况以及启动子功能元件,为后续进一步研究FPS在刺五加中的表达调控奠定了基础。
In order to obtain the DNA and promoter sequence of FPS and its functional elements information and gene structure, primers were designed according to the cloned FPS cDNA sequence, the genomic DNA and promoter sequence of FPS were cloned by TAIL-PCR technique, Bio-software analyzes gene structure and promoter function. The full-length FPS gene of Acanthopanax senticosus was 7 952 bp in length, containing 12 exons and 11 introns. The promoter sequence contained 25 types of functional elements, including 62 TATA-boxes, 24 CAAT-boxes, And other types of important features 34. In this study, we cloned the DNA sequence of FPS and the full length of the promoter for the first time, and obtained the distribution of exon and exon, as well as the functional elements of promoter, which laid the foundation for the further study on the regulation of FPS expression in Acanthopanax.