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截叶毛白杨是从毛白杨中选出的一个优良变种,繁殖较困难,供不应求,为此开展了组织培养试验。通过三年来的室内外试验,筛选出了植物生长素萘乙酸的使用浓度以0.2～0.3毫克/升、细胞激动素腺苷的使用浓度以0.25～0.05毫克/升为最佳。用正交试验的方法,选出分化茎根最优的搭配是米勒培养基+腺苷0.05毫克/升+萘乙酸0.2毫克/升。简化培养基试用来源广泛、价格便宜的马铃薯和玉米,代替 MS 培养基中五种大量元素及九种微量元素和维生素等,用蛭石作基质减少了琼脂用量的70%。组培苗质量较好。选用优良无性系作继代培养的外植体,一片叶带一个腋芽接种在上述培养基上一次分化成完整的幼苗,在温室及室外日光下培养。经日光下锻炼,幼苗茎杆由绿色变为棕红色时栽植成活较好。试用组培苗的干重/鲜重×100%作为木质化程度的指标。指标高的幼苗栽植成活率高,指标低的成活率低。经试验在12个月内均可栽植,但冬天要栽在温室。
Populus tomentosa is a fine variant selected from Populus tomentosa, breeding more difficult, in short supply, for this to carry out tissue culture experiments. Through the three years of indoor and outdoor experiments, the use of plant growth hormone naphthalene acetic acid concentration of 0.2 to 0.3 mg / L, the use of cytokinin concentration of 0.25 to 0.05 mg / L is the best. Orthogonal test method, the best choice of differentiation stem root mix is Miller’s medium + adenosine 0.05 mg / L + NAA 0.2 mg / L. Simplify the culture medium Extensive, affordable potatoes and maize were used in experiments to replace the five macronutrients and nine trace elements and vitamins in the MS medium. Using vermiculite as a substrate reduced the amount of agar by 70%. Tissue culture better quality. Selection of excellent clones for subculture explants, a leaf axillary bud inoculated on the above medium once differentiated into intact seedlings, in the greenhouse and outdoor daylight culture. After daylight training, seedling stalks from green to brown red planting better survival. Trial dry weight of tissue culture seedlings × 100% as an indicator of the degree of lignification. High seedling planted high survival rate, low indicator of low survival rate. The test can be planted in 12 months, but in winter to plant in the greenhouse.