Organic matter and iron and maganese oxides have been considered as the major affecting factors for metals in anoxic or oxidized sediment. In recent research, clay and sulfide are found as major factors in oxic or oxidized sediments that might affect bioavailability of metals. To test this hypothesis, the influence of sulfide, measured as acid-volatile sulfide (AVS), and clay content on the bioavailability of zinc and cadmium in sediments was examined. Laboratory simulative experiment and field verification experiment were conducted, showing that the bioavailability of zinc and cadmium is strongly correlated to AVS and clay content in sediments. Taking into account both AVS and clay parameters in sediments together can better indicate the bioavailability of zinc and cadmium rather than considering one of them alone. Organic matter and iron and maganese oxides have been considered as the major affecting factors for metals in anoxic or oxidized sediment. In recent research, clay and sulfide are found as major factors in oxic or oxidized sediments that might affect bioavailability of metals. To test this hypothesis, the influence of sulfide, measured as acid-volatile sulfide (AVS), and clay content on the bioavailability of zinc and cadmium in sediments was examined. Taking into account both AVS and clay parameters in sediments together can be indicative of the bioavailability of zinc and cadmium rather than considering one of them alone.