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Trager等在1976年建立的恶性疟原虫体外连续培养的技术虽已广泛应用于疟疾研究,但尚未用猴类红细胞进行培养。为解决此问题并改进恶性疟原虫-松鼠猴的实验模型,作者试用松鼠猴红细胞培养恶性疟原虫。所用的恶性疟原虫为乌干达FUP株和IndoⅠ株,均已适应于松鼠猴体内生长。不感染的红细胞及血清取自切脾前后的同一只雌性健康松鼠猴。试验用的人A型和AB型血清系经松鼠猴红细胞吸附而无凝集者。
Trager and other established in 1976, Plasmodium falciparum continuous culture in vitro technology has been widely used in malaria research, but has not yet been cultured with monkey erythrocytes. To solve this problem and to improve the experimental model of P. falciparum-squirrel monkey, the authors experimented with squirrel monkey erythrocytes to culture P. falciparum. Plasmodium falciparum used were Uganda FUP strain and Indo strain, both adapted to squirrel monkey growth. Non-infected red blood cells and serum were taken from the same female healthy squirrel monkey before and after splenectomy. The tested human type A and type AB sera were adsorbed by squirrel monkey erythrocytes without agglutination.