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目的研究芹菜素对哮喘模型小鼠肺组织OX40表达、气道炎症和气道高反应性的干预作用。方法 24只SPF级BALB/c小鼠随机均分为正常组、哮喘组、芹菜素组和布地奈德组。采用卵白蛋白(OVA)致敏和激发建立哮喘模型。末次激发24h后,测定气道反应性,HE染色观察炎症细胞浸润,ELISA法分别检测血清总IgE、OVA特异性IgE(OVA-sIgE)以及支气管肺泡灌洗液(BALF)中IL-4和IL-13的含量。Western blot检测肺组织OX40蛋白的表达。结果随着氯化乙酰胆碱浓度的增加,哮喘组气道阻力明显增加,正常组仅轻度增加,芹菜素组和布地奈德组气道阻力的增加程度低于哮喘组(P<0.05)。哮喘组BALF中炎症细胞总数、嗜酸性粒细胞计数、IL-4和IL-13水平、血清总IgE和OVA-sIgE水平以及肺组织OX40表达水平均高于正常组(P<0.05);芹菜素组和布地奈德组上述指标均低于哮喘组(P<0.05)。结论芹菜素可抑制哮喘模型小鼠气道炎症和气道高反应性,可能与抑制OX40/OX40配体协同刺激通路有关。
Objective To study the effects of apigenin on lung tissue OX40 expression, airway inflammation and airway hyperresponsiveness in asthmatic mice. Methods Twenty-four SPF BALB / c mice were randomly divided into normal group, asthma group, apigenin group and budesonide group. The model of asthma was established by ovalbumin (OVA) sensitization and challenge. After the last challenge, the airway reactivity was measured, the infiltration of inflammatory cells was observed by HE staining, the levels of total serum IgE, OVA-specific IgE (OVA-sIgE), and bronchoalveolar lavage fluid (BALF) -13 content. Western blot detection of OX40 protein expression in lung tissue. Results As the concentration of acetylcholine increased, the airway resistance in asthma group increased significantly, while that in normal group increased only slightly. The increase of airway resistance in apigenin group and budesonide group was lower than that in asthma group (P <0.05). The total number of inflammatory cells, eosinophil counts, IL-4 and IL-13 levels, serum total IgE and OVA-sIgE levels, and lung tissue OX40 expression levels in BALF in asthma group were higher than those in normal group (P <0.05) The above indexes in group and budesonide group were lower than those in asthma group (P <0.05). Conclusions Apigenin can inhibit airway inflammation and airway hyperresponsiveness in asthmatic mice and may be related to the inhibition of the co-stimulatory pathway of OX40 / OX40 ligands.